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| 电 话： |
021-68610355 |
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021-68610355 |
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021-68610299 |
| 传 真： |
021-50353995 |
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| 落射荧光显微镜 E200 |
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| 全反射显微系统（消逝波图像系统） |
| The above image shows the basal plane of ECV304cells expressing the fusion protein GFP-AP-2.AP-2 is an adapter protein that localizes with clathrin coated pits ,The fluorescence emission (bright spots in the image) correlates with the number of pits observed. The edges of the cell are indicated by the dotted line and the arrows indicates sing molecules of GFP-AP-2 localized at the cell membrane. The scale bar is 1μm. |
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| Enables Visualization of Single Molecule Fluorescence |
A TIRF(Total Internal Reflection Fluorescence) microscopy system utilizes evanescent wave illumination. This illumination causes an extremely thin section of fluorophor excitation (typically less than 100nm) resuting in high-contrast real time imaging of labeled molecules at the cell membrane or basal plane of the specimen.
The unique extendible configuration of the Nikon TE2000 microscope enables simultaneous mounting of both TIRF and epi-fluorescence illumination systems, which allows easy switching between the two, or image overlay.
The dedicated TIRF objective(60X 1.45N.AA)has a correction collar for cover-glass thickness calibrated at 23℃(room temperature) or 37℃ (physiological temperature). This feature allows for correction in temperature-induced changes in refractive index of immersion oil that could induce spherical aberration. |
| Main Specifications
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Microscope Unit:
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Nikon Inverted Microscope TE2000 (with epi-fluorescence attachment)
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Dedicated objectives:
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CFI Plan Apo TIRF 60X/1.45 oil,
CFI Plan Apo TIRF 100X/1.45 oil
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Direct C-mount TV adapter
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Evanescent Wave Illumination Unit:
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Laser: blue (488), green (532), red (633), up to 3 types can be used
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Provided with filter cassette (dichroic mirror/barrier filter)
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Aperture diaphragm with adjustment mechanism
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ND filters (ND2, ND8, ND32)
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